Workshop Description

Real-time PCR (also known as quantitative PCR or qPCR) is the simultaneous amplification and detection/quantification of nucleic acids using the polymerase chain reaction (PCR). It can precisely distinguish and measure specific nucleic acid sequences in a sample even if there is only a very small quantity and monitor the amplification progress using fluorescent technology. qPCR offers sensitivity, specificity, and wide dynamic range for detecting target nucleic acids, making it a useful and powerful technology for research applications that cover the spectrum of biology from basic research to translational medicine to applied biology.

This workshop will focus on the basic concepts, chemistries, and instrumentation of real-time PCR, workflow, optimization, troubleshooting and include present applications and future perspectives for this technology in biomedical sciences and in life science education.

Content

• What is real-time PCR used for and how does it work.

• Homogenous fluorescent chemistries for Real-time PCR

• What does real-time data look like.

• Workflow and sample preparation for Analysis by qPCR.

• Guidelines for the design of a reliable real-time PCR Assay.

• The validation criteria of Real-time PCR Assays.

• Detection and diagnosis of infectious agents.

• Monitoring cancer patients response to chemotherapy.

• Analysis of mRNA Expression.

• Genotyping of Single Nucleotide Polymorphisms.

• Genetic Alterations Linked to infertility.