Course Structure

The fertilization of human oocytes outside the body, the culturing of the embryo (s) in the laboratory, and the subsequent transfer of embryo (s) to the uterus has resulted in the birth of tens of thousands of children worldwide. For most couples, Assisted Reproductive Technologies (ARTs) are the last, best option for having a child, and often occur after sustained treatment failure with tremendous emotional, physical, and financial cost Genetic testing for embryos is a controversial issue but one thing is for certain when women use this screening process – especially older women and those who have  repeat miscarriages – are less apt to miscarry implanted embryos and data from recent studies show that certain methods for genetic screening show significantly higher IVF success rates

This training allows trainees to understand the basic life cycle of the genetic traits carried by the sperm by focusing on the errors in meiosis and abnormalities in spermatogenesis/spermatogenesis. Furthermore, we will focus on human chromosome structure, methodology, and techniques for the visualization of chromosome aberrations that lead to abnormalities in embryos and also can cause infertility

Laboratory Work

• Basic semen analysis.

• Morphology staining and sperm Morphology Evaluation.

• Sperm Chromatin Dispersion Assay for evaluation of Sperm DNA integrity.

• Sperm preparation techniques for different ART procedures. 

• Handling of different types of media, aliquoting, and further storage. 

• Pre Implementation Genetic Diagnosis - PGD.

• Male infertility and Y-chromosome microdeletions.

• Chromosomal abnormalities in couples with a history of genetic disorders.